synapsin ii (Alomone Labs)
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90
Structured Review
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synapsin ii
Synapsin Ii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synapsin ii/product/Alomone Labs
Average 90 stars, based on 2 article reviews
Synapsin Ii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synapsin ii/product/Alomone Labs
Average 90 stars, based on 2 article reviews
synapsin ii - by Bioz Stars,
2026-04
90/100 stars
Images
1) Product Images from "GABA-induced Ca 2+ signaling in the primary cilium of neurons"
Article Title: GABA-induced Ca 2+ signaling in the primary cilium of neurons
Journal: bioRxiv
doi: 10.1101/2025.05.26.656109
Figure Legend Snippet: A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive for AC3 (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses (Synapsin II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Techniques Used: Immunofluorescence, Expressing, Cell Culture, Activation Assay, Activity Assay, Control, Two Tailed Test, MANN-WHITNEY, Fluorescence
